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J. KIMS Technol > Volume 14(4); 2011 > Article
Journal of the Korea Institute of Military Science and Technology 2011;14(4):710-718.
DOI: https://doi.org/10.9766/KIMST.2011.14.4.710   
Purification and Characterization of Recombinant Anthrax Edema Factor
Yu-Gene Kim
부종요소 단백질의 정제 및 특성분석 연구
김유진
국방과학연구소 5본부 4부
Abstract
Edema factor(EF) is a portion of anthrax toxin which produces edema when combined with protective antigen. This paper describes about technique for cloning, expression, purification and activity test of EF. Using the E. coli expression system, we could make recombinant EF protein although it's origin is Bacillus anthracis. And also we could culture massively and purify highly pure protein. Finally we confirm a enzyme activity of purified EF to increase intracellular cAMP level. Through establishing this technique, it can be possible to research about EF in depth and apply to expression and purification of many other protein in biology.
Key Words: Anthrax, EF(Edema Factor)
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