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Journal of the Korea Institute of Military Science and Technology 2004;7(3):110-121.
Characteristics study of biological materials using pyrolysis-mass spectrometry
Sun-Kyung Choi
Agency for Defense Development
열분해 질량분석법을 이용한 생물학 물질의 특성 연구
최선경
국방과학연구소
Abstract
Pyrolysis-mass spectrometry, incorporating an in situ thermal hydrolysis and methylation(THM) step, has been used to study biological materials for bacteria, toxin and virus. Newly developed pyrolyzer was used to decompose biological materials, and tetramethylammonium hydroxide(TMAH) was used as a methylation reagent. Chemical ionization(CI) using ethanol and ion trap mass spectrometer(ITMS) were used to ionize and analyze of pyrolysis components, respectively. Analytical characteristics of bacteria (including spore), virus and toxin were analyzed. Also acquisition and interpretation of mass spectra as biomarkers for classification/identification of biological material s were explained.
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