Discovery of Protein Biomarkers for Infected Bacillus anthracis Spores in Using Proteomic Analysis

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Journal of the Korea Institute of Military Science and Technology 2004;7(1):77-81.

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프로테오믹스를 이용한 탄저균 아포 감염에 대한 바이오마커 탐색

서귀문, 남덕화, 오광근, 김성주, 김지천, 채영규

¹한양대학교 생화학 및 분자생물학과
²(주)셀엔젠

Abstract

The etiological agent is Bacillus anthracis, a gram-positive rod-shaped bacterium able to form spores. In order to elucidate the mechanism of infecttion on human macrophage cells, we performed two-dimensional electrophoresis and MALDI-TOF analysis using the infected human macrophage cells with the spores of B. anthracis Sterne of inactivated B. anthracis Sterne. We identified 9 proteins which related to the infection of Bacillus anthracis spores on human macrophage cells at the early stage events. Maybe nine proteins will be bio-markers and vaccine candidates to the Bacillus anthracis spore infection.