Development and Validation Study of Biological Agent Detection Kit

Joe, Hae Eun

doi:2019.22.4.575

J. KIMS Technol > Volume 22(4); 2019 > Article

Research Paper

Journal of the Korea Institute of Military Science and Technology 2019;22(4):575-580.
DOI: https://doi.org/10.9766/KIMST.2019.22.4.575

Development and Validation Study of Biological Agent Detection Kit

Hae Eun Joe

The 4th Research and Development Institute, Agency for Defense Development

생물학작용제 검출 키트 개발 및 성능시험 연구

조혜은

국방과학연구소 제4기술연구본부

Abstract

In biological warfare, it is important to identify biological agents for proper treatment. We focused on developing a real-time RT-PCR kit that can detect multiple species of biological agents. AccuPower(R) Biothreat Real-Time RT-PCR Kit(v3.0) could detect Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Francisella tularensis, Salmonella typhi, Rickettsia prowazekii, Variola virus, Hantaan virus, Yellow fever virus, Brucella spp., Shigella dysenteriae in a single reaction. The results showed that the kit was verified to be able to detect at least 0.005 ng of nucleotide and 10,000 CFU/ml of bacteria. Therefore, the kit is expected to be used as a rapid and sensitive detection kit for 11 species of biological agents within 2 hours.

Key Words: Real-Time RT-PCR Kit, Biological Agents, Detection