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J. KIMS Technol > Volume 20(5); 2017 > Article
Journal of the Korea Institute of Military Science and Technology 2017;20(5):726-732.
DOI: https://doi.org/10.9766/KIMST.2017.20.5.726   
A Study on the Validation system of Detection for Biological Agents Using Real-Time PCR
Younggil Cha, Bonwoo Koo, Seongjoo Kim, Namil Kim, Hanoh Park
1Molecular Diagnostic Division, Bioneer Corp.
2The 5th Research and Development Institute, Agency for Defense Development
실시간 중합효소 연쇄반응을 활용한 생물작용제 검증시스템 연구
차영길, 구본우, 김성주, 김남일, 박한오
1(주)바이오니아 진단과학시약연구소
2국방과학연구소 제5기술연구본부
Abstract
Bacillus anthracis, Vibrio cholerae, Variola virus and Shigella dysenteriae are classified as category A and B biological weapons. In this study suggest that 4 genes of Bacillus anthracis, 2 genes of Vibrio cholerae, 1 gene of Variola virus and 1 gene of Shigella dysenteriae were detective 50~500 fg of target DNA per reaction using real-time PCR based assay. Also analytical specificity did not show any cross-reactivity with other related bacteria. Reliable and one reaction could be effective early diagnostic and treatment for detection of unknown samples.
Key Words: Bacillus Anthracis, Vibrio Cholerae, Shigella Dysenteriae, Variola Virus, Real-Time PCR, Bacillus Anthracis, Vibrio Cholerae


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