A Study on the Validation system of Detection for Biological Agents Using Real-Time PCR |
Younggil Cha, Bonwoo Koo, Seongjoo Kim, Namil Kim, Hanoh Park |
1Molecular Diagnostic Division, Bioneer Corp. 2The 5th Research and Development Institute, Agency for Defense Development |
실시간 중합효소 연쇄반응을 활용한 생물작용제 검증시스템 연구 |
차영길, 구본우, 김성주, 김남일, 박한오 |
1(주)바이오니아 진단과학시약연구소 2국방과학연구소 제5기술연구본부 |
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Abstract |
Bacillus anthracis, Vibrio cholerae, Variola virus and Shigella dysenteriae are classified as category A and B biological weapons. In this study suggest that 4 genes of Bacillus anthracis, 2 genes of Vibrio cholerae, 1 gene of Variola virus and 1 gene of Shigella dysenteriae were detective 50~500 fg of target DNA per reaction using real-time PCR based assay. Also analytical specificity did not show any cross-reactivity with other related bacteria. Reliable and one reaction could be effective early diagnostic and treatment for detection of unknown samples. |
Key Words:
Bacillus Anthracis, Vibrio Cholerae, Shigella Dysenteriae, Variola Virus, Real-Time PCR, Bacillus Anthracis, Vibrio Cholerae |
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